期刊
JOURNAL OF INTERNATIONAL MEDICAL RESEARCH
卷 44, 期 3, 页码 419-432出版社
SAGE PUBLICATIONS LTD
DOI: 10.1177/0300060516630852
关键词
Intracerebral haemorrhage; peripheral blood; haematoma; microRNA; microRNA polymerase chain reaction array; has-miR-21-5p
资金
- National Natural Science Foundation of China [81271291]
Objective To measure the differential expression of microRNAs (miRNAs) in peripheral blood samples from patients with intracerebral haemorrhage (ICH) and to measure the levels of hsa-miR-21-5p in peripheral blood and haematoma samples from patients with ICH. Methods This case-control study enrolled individuals with ICH in the putamen treated by craniotomy and age- and sex-matched healthy control subjects. Serum miRNA expression profiles were determined in the patient and control groups using miRNA polymerase chain reaction (PCR) arrays. The ICH-related miRNA hsa-miR-21-5p was selected and its differential expression was assessed in peripheral blood and haematoma specimens from patients with ICH compared with peripheral blood samples controls using real-time PCR. Results Seven patients and five control subjects were included in the miRNA expression profile analysis; and 31 patients and 22 control subjects provided samples for the real-time PCR of hsa-miR-21-5p expression. A total of 59 miRNAs were significantly downregulated in patients with ICH. Relative hsa-miR-21-5p levels of 0.43 and 0.31 for peripheral blood and haematoma samples, respectively, were obtained in the patient group compared with the control subjects. Conclusion Hsa-miR-21-5p levels were significantly reduced in both peripheral blood and haematoma samples in patients with ICH.
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