期刊
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
卷 219, 期 -, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.jpba.2022.114946
关键词
Florfenicol; Immunochromatographic strips; Rapid on -site detection; Carboxylated fluorescent microsphere
资金
- Science and Technology Project of Guangzhou Municipal Market Supervision Administration, China [2021KJ53]
- Start -Up Grant of the Southern Medical University, China [G620522046]
- Science and Technology Program of Guangzhou, China [202102021000]
- Medical Scientific Research Foundation of Guangdong Province of China, China [A2022200]
- GuangDong Basic and Applied Basic Research Foundation, China [2021A1515111062]
- Science and Technology Project of Guangdong Administration for Market Regulation, China [2022CS02]
A sensitive and quantitative on-site detecting solution, monoclonal antibody-based carboxylated fluorescent microsphere immunochromatographic test strip assay (FM-ICTS), is proposed for the detection of florfenicol (FF) in eggs. The method demonstrates sensitivity in detecting low concentrations of FF, quantitatively measures its concentration, and exhibits high repeatability and measurement accuracy. Additionally, the test strip demonstrates high stability and can be stored for an extended period without loss of detection ability.
Florfenicol (FF), used popularly in prevention and treatment of virus infections in livestock and poultry, has widely been found in eggs and harmful to human health. In this work, a sensitive and quantitative on-site detecting solution, monoclonal antibody-based carboxylated fluorescent microsphere immunochromatographic test strip assay (FM-ICTS), is design and applied for FF detection. The proposed method can sensitively detect FF in low detection limit of 0.030 ng/g and quantitatively measure its concentration from 0.1 ng/mL to 8.1 ng/mL (R-2 = 0.9991) with high repeatability (CV < 8.0 %). In addition, the established FM-ICTS method exhibited high measurement accuracy in FF samples as compared with HPLC-MS analysis and demonstrated satisfied recoveries (99.1-101.3 %). More importantly, the quantitative FF test strip demonstrate ultra-high stability, which presents approximately equivalent detection ability to the fresh one after stored at 4 ?C for more than one year or stored at 37 ?C for 60 days. Therefore, the proposed method is a promising solution for rapidly and sensitively quantitative determination of FF in eggs.
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