4.7 Article

Combining FT-IR spectroscopy and multivariate analysis for qualitative and quantitative analysis of the cell wall composition changes during apples development

期刊

CARBOHYDRATE POLYMERS
卷 115, 期 -, 页码 93-103

出版社

ELSEVIER SCI LTD
DOI: 10.1016/j.carbpol.2014.08.039

关键词

Plant cell wall; Polysaccharides; FT-IR; Spectroscopic analysis; Chemometry

资金

  1. Polish National Centre of Science (NCN) [2011/01/D/NZ9/02494]

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The aim of this work was to quantitatively and qualitatively determine the composition of the cell wall material from apples during development by means of Fourier transform infrared (FT-IR) spectroscopy. The FT-IR region of 1500-800 cm(-1), containing characteristic bands for galacturonic acid, hemicellulose and cellulose, was examined using principal component analysis (PCA), k-means clustering and partial least squares (PLS). The samples were differentiated by development stage and cultivar using PCA and k-means clustering. PLS calibration models for galacturonic acid, hemicellulose and cellulose content from FTIR spectra were developed and validated with the reference data. PLS models were tested using the root-mean-square errors of cross-validation for contents of galacturonic acid, hemicellulose and cellulose which was 8.30 mg/g, 4.08% and 1.74%, respectively. It was proven that FT-IR spectroscopy combined with chemometric methods has potential for fast and reliable determination of the main constituents of fruit cell walls. (C) 2014 Elsevier Ltd. All rights reserved.

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