期刊
JOURNAL OF NUTRITIONAL BIOCHEMISTRY
卷 108, 期 -, 页码 -出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.jnutbio.2022.109085
关键词
EGCG; Obesity; Precocious puberty; Sex hormones; VO
资金
- National Natural Science Foundation of China [81773407]
This study aimed to investigate the potential regulatory pathways of EGCG in preventing obesity-related precocious puberty. The results showed that EGCG could prevent precocious puberty through the NKB/NK3R signaling pathway.
This study aimed to explore the potential regulatory pathways of (-)-epigallocatechin-3-gallate (EGCG) in preventing obesity-related precocious puberty. A retrospective analysis on the impact of EGCG on puberty onset in obese girls was conducted on plasma samples collected from a human randomized controlled trial. In the trial, participants consumed EGCG capsules for 12 weeks. In the animal experiment, rats were divided into four groups: normal diet control (NC) group, high-fat diet (HFD) group, NC + EGCG group, and HFD + EGCG group. Blood samples were collected on postnatal days 27, 33, and 36 to detect sexual development indicators. The hypothalamic expressions of kisspeptin/Kiss1R and neurokinin B (NKB)/NK3R signaling were measured by RT-qPCR and Western blot assay. The ovary NKB protein expression was assessed by immunohistochemical assays. Serum NKB level in the EGCG group was lower than the placebo group by 0.599 ng/mL [beta= -0.599, 95% CI: (-1.005, -0.193)], at the end of intervention and after adjusting for confounders (clinical study). In the animal experiment, EGCG intervention could significantly delay the vaginal opening (VO) time of rats fed with HFD. On day 33, EGCG intervention could significantly reduce serum NKB, luteinizing hormone (LH) levels, ovarian NKB protein expression, and endometrial thickness of HFD-fed rats, while EGCG intervention could remarkably increase mRNA and protein expression of NKB/NK3R. EGCG could prevent obesity-related precocious puberty through NKB/NK3R signaling pathway, which may provide a novel insight into the role of EGCG in preventing precocious puberty in obese girls. (c) 2022 Elsevier Inc. All rights reserved.
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