期刊
CARBOHYDRATE POLYMERS
卷 133, 期 -, 页码 596-604出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.carbpol.2015.07.031
关键词
Polysaccharide; Cyclocarya paliurus; Acetylation; Physicochemical; Characterization; Antioxidant activity
资金
- National Natural Science Foundation of China [31471702, 31201297, 31130041]
- National Key Technology R AMP
- D Program of China [2012BAD33B06]
- Major Research plan of the Natural Science Foundation of Jiangxi province, China [20152ACB21004]
- Science Funds of Educational Commission of Jiangxi Province, China [GJJ14144]
- Objective-Oriented Project of State Key Laboratory of Food Science and Technology, Nanchang University, China [SKLF-ZZA-201301]
In this study, polysaccharides extracted from Cyclocarya paliurus leaves were modified to obtain its three acetylated derivatives, Ac-CP1, Ac-CP2, and Ac-CP3. The physicochemical characteristics and antioxidant activities of acetylated derivatives were investigated. The results of chemical and FT-IR spectrum analysis showed differences between acetylated derivatives and native C. paliurus polysaccharide, which revealed that the acetylation were successful. Relative to unmodified polysaccharide, the protein contents of acetylated derivatives decreased, while carbohydrate values increased. The molecular weight (Mw) of acetylated derivatives were approximately 1.05-1.09 x 10(6) Da and were mainly composed of Ara, Gal, Glc, Man, GalA. Ac-CP1 with relatively low degree of substitution (0.13 +/- 0.01) exhibited excellent antioxidant activity in DPPH radical assay (95.21 +/- 0.89%), and also had strong chelating activity on P-carotene linoleic acid assay (34.64 +/- 2.07%) at 0.5 mg/ml. In addition, scanning electron microscope (SEM) observations suggested that acetylation could change the morphology and structure of polysaccharides from C. paliurus leaves. (C) 2015 Elsevier Ltd. All rights reserved.
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