期刊
JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY
卷 32, 期 10, 页码 1245-1252出版社
KOREAN SOC MICROBIOLOGY & BIOTECHNOLOGY
DOI: 10.4014/jmb.2208.08042
关键词
iPSC; LCD; reprogramming; in vitro; natural compound; MET
资金
- National Research Foundation of Korea (NRF) - Ministry of Education [NRF-2021R111A1A01048981]
- Korea government (MSIP
- Ministry of Science, ICT & Future Planning) [NRF-2017R1C1B5077043]
In this study, it was demonstrated that Licochalcone D (LCD) can improve the efficiency of induced pluripotent stem cell (iPSC) reprogramming and promote mesenchymal-to-epithelial transition (MET) during the early stages of reprogramming.
Induced pluripotent stem cells (iPSCs) can be generated from somatic cells using Oct4, Sox2, Klf4, and c-Myc (OSKM). Small molecules can enhance reprogramming. Licochalcone D (LCD), a flavonoid compound present mainly in the roots of Glycyrrhiza inflata, acts on known signaling pathways involved in transcriptional activity and signal transduction, including the PGC1-alpha and MAPK families. In this study, we demonstrated that LCD improved reprogramming efficiency. LCD-treated iPSCs (LCD-iPSCs) expressed pluripotency-related genes Oct4, Sox2, Nanog, and Prdm14. Moreover, LCD-iPSCs differentiated into all three germ layers in vitro and formed chimeras. The mesenchymal-to-epithelial transition (MET) is critical for somatic cell reprogramming. We found that the expression levels of mesenchymal genes (Snail2 and Twist) decreased and those of epithelial genes (DSP, Cldn3, Crb3, and Ocln) dramatically increased in OR-MEF (OG2+/+/ROSA26+/+) cells treated with LCD for 3 days, indicating that MET effectively occurred in LCD-treated OR-MEF cells. Thus, LCD enhanced the generation of iPSCs from somatic cells by promoting MET at the early stages of reprogramming.
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