4.3 Article

Detecting aquatic pathogens with field-compatible dried qPCR assays

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JOURNAL OF MICROBIOLOGICAL METHODS
卷 202, 期 -, 页码 -

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ELSEVIER
DOI: 10.1016/j.mimet.2022.106594

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Lyophilization; qPCR; Air-dried assay; Environmental diagnostics; Aquatic pathogens; eDNA

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Field-ready qPCR assays with extended shelf-life are developed and tested for monitoring emerging aquatic pathogens, enabling quick conservation and management decisions.
Field-ready qPCR assays with extended shelf-life support monitoring programs for emerging aquatic pathogens and enable quick conservation and management decisions. Here, we developed, validated, and tested the shelf life of qPCR assays targeting Gyrodactylus salaris and Aphanomyces astaci with lyophilization and air-drying.

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