Sensitive determination of ascorbic acid, dopamine and uric acid by glassy carbon electrodes modified with β-cyclodextrin and graphene oxide

In this paper, an ultra-sensitive glassy carbon electrode modified with beta-cyclodextrin and graphene oxide (beta-CD-GO/GCE) has been prepared for the detection of biomolecules [ascorbic acid (AA), dopamine (DA) and uric acid (UA)] in aqueous solution at mu M level. The three biomolecules (AA, DA and UA) can be simultaneously detected by the modified electrodes, and the electrical signals corresponding to various biomolecules can be identified clearly in cyclic voltammetry and differential pulse voltammetry curves. The simultaneous detection limits of AA, DA, UA were 4.58, 1.56, 1.27 mu M, respectively. This ultra-sensitivity should be attributed to the formation of host-guest complexes between beta-CD and the biomolecules. Furthermore, the anti-interference experiments indicated the beta-CD-GO/GCE exhibited satisfactory selective recognition performance.

Automated summary

In this study, an ultra-sensitive electrode modified with beta-cyclodextrin and graphene oxide was successfully prepared for the detection of biomolecules in aqueous solutions. Simultaneous detection of three biomolecules (ascorbic acid, dopamine, and uric acid) was achieved, and the electrical signals corresponding to each biomolecule were clearly identified using cyclic voltammetry and differential pulse voltammetry. The ultra-sensitivity of the electrode can be attributed to the formation of host-guest complexes between beta-cyclodextrin and the biomolecules. Furthermore, the modified electrode showed satisfactory selective recognition performance based on anti-interference experiments.