A cost-effective tsCUT&Tag method for profiling transcription factor binding landscape

Knowledge of the transcription factor binding landscape (TFBL) is necessary to analyze gene regulatory networks for important agronomic traits. However, a low-cost and high-throughput in vivo chromatin profiling method is still lacking in plants. Here, we developed a transient and simplified cleavage under targets and tagmentation (tsCUT&Tag) that combines transient expression of transcription factor proteins in protoplasts with a simplified CUT&Tag without nucleus extraction. Our tsCUT&Tag method provided higher data quality and signal resolution with lower sequencing depth compared with traditional ChIP-seq. Furthermore, we developed a strategy combining tsCUT&Tag with machine learning, which has great potential for profiling the TFBL across plant development.

Automated summary

A transient and simplified tsCUT&Tag method was developed for high-throughput chromatin profiling in plants. This method provided higher data quality and signal resolution compared to traditional ChIP-seq, and when combined with machine learning, it has great potential for profiling the transcription factor binding landscape during plant development.