Integrating transcriptome and physiological analysis to reveal the essential responses of Daphnia magna to antimony trioxide nanoparticle

Antimony (Sb) pollution has already posed a severe threat to the aquatic ecosystem. However, the toxicity mechanisms of Sb on aquatic organisms are far from being elucidated. One of the crucial questions remaining unresolved is the characterization of molecular toxicity of Sb(III). Transcriptomics profiling combined with physiological characterizations was applied to investigate the response of Daphnia magna to nano-size antimony trioxide (nATO) and its soluble Sb(III) counterpart antimony potassium tartrate (APT) in the present study. Both nATO and APT induced the formation of oxidative stress, enhanced the activities of anti-oxidative enzymes, altered the metabolism of xenobiotics, increased the concentration of hydrogen sulfide (H2S) and nitric oxide (NO), and triggered the self-protection mechanisms such as ubiquitin-mediated proteolysis. In addition, nATO and APT caused damage to the nervous system of D. magna, inhibited its locomotion and nutrient uptake in a concentration-dependent manner. Moreover, nATO exposure enhanced the autophagy activity, reflected by the up-regulated expression of hypoxia-inducible factor-1 alpha, calmodulin-dependent protein kinase-beta, and inositolrequiring enzyme 1. The present study, for the first time, depicted a global map of cellular response to nATO, provided essential information on Sb(III) toxicity to aquatic organisms, and is of great significance to the development of Sb management strategies.

Automated summary

This study investigated the toxicity mechanisms of nano-size antimony trioxide (nATO) and soluble Sb(III) compounds on Daphnia magna. The results showed that both nATO and soluble Sb(III) induced oxidative stress, altered metabolism, and caused damage to the nervous system of D. magna, indicating their toxicity to aquatic organisms.