期刊
JOURNAL OF HAZARDOUS MATERIALS
卷 438, 期 -, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.jhazmat.2022.129511
关键词
Cadmium; Flavonoids; Hydrogen peroxide; Phytotoxicity; RBOH1
资金
- Key Research and Development Program of Zhejiang Province [2021C02040, 2021C02052]
- National Key R&D Program of China [2020YFD10007, 2018YFD1000800]
- Innovation Project of the Chinese Academy of Agricultural Sciences [CAAS-ASTIP-2019-TRICAAS]
- China Agriculture Research System of MOF and MARA [CARS-23-G44]
- State Key Laboratory Breeding Base for the Zhejiang Sustainable Pest and Disease Control [2010DS700124ZZ1903]
- National Natural Science Foundation of China [31950410555, 32172555]
- Ministry of Science and Technology of the People's Republic of China [QNJ20200226001, QNJ2021026001]
- Zhejiang Provincial Major Agricultural Science and Technology Projects of New Varieties Breeding [2021C02065]
- Henan Engineering Technology Research Center for Horticultural Crop safety and Disease Control
- Henan International Joint Laboratory of Stress Resistance Regulation and Safe Production of Protected Vegetables
The application of EECCG alleviated the adverse effects of cadmium stress in tomato plants by reducing oxidative stress and promoting the expression of RBOH1 gene. These findings contribute to our understanding of the stress resistance properties of EGCG in plants.
Toxic heavy metal cadmium (Cd) reduces crop yield and threatens human health via the food chain. The bioactive flavonoid Epigallocatechin-3-gallate' (EGCG) affects plant stress response; however, the function of EGCG in Cd tolerance and the molecular pathways remain largely unknown. Here, we revealed that root application of EGCG alleviated Cd stress in tomato plants. While Cd stress decreased Fv/Fm, ?(PSII), photosynthetic rate, root growth, root vitality and biomass accumulation by increasing reactive oxygen species (ROS) accumulation and lipid peroxidation, exogenous EGCG minimized excessive ROS accumulation and oxidative stress by promoting the activity of antioxidant enzymes and redox poise in roots and leaves. Moreover, EGCG induced the transcript of RESPIRATORY BURST OXIDASE HOMOLOG1 (RBOH1) and decreased Cd content and photoinhibition in leaves. Interestingly, similar to EGCG, exogenous H2O2 application also enhanced Cd tolerance; however, the application of an NADPH oxidase inhibitor, diphenyleneiodonium (DPI), aggravated Cd phytotoxicity and attenuated the beneficial effects of EGCG on plant tolerance to Cd stress, suggesting that root applied EGCG-induced expression of RBOH1 and associated H2O2 signaling mediate the EGCG-induced enhanced Cd tolerance. This work elucidates a fundamental mechanism behind EGCG-mediated Cd tolerance and contributes to our existing knowledge of stress resistance properties of EGCG in plants.
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