期刊
JOURNAL OF HAZARDOUS MATERIALS
卷 439, 期 -, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.jhazmat.2022.129675
关键词
Microbial-induced phosphate precipitation; Pb bioimmobilization; Penicillium oxalicum; Metabolomics; Transcriptomics
资金
- Outstanding Youth Funding of Hunan Education Department [2019112500702]
- Huxiang High -Level Talents Gathering Project for Innovative Talents [2018RS3090]
This study investigated the metabolic changes in PSM Penicillium oxalicum postexposure to Pb2+ and/or tricalcium phosphate (TCP). The addition of TCP improved Pb mineralization, antioxidative enzyme activities, and metabolic response in P. oxalicum. The results provided new insights into the biotoxicity of Pb2+ exposure to microorganisms in the MIPP process.
Microbial-induced phosphate (P) precipitation (MIPP) based on P-solubilizing microorganisms (PSM) is regarded as a promising approach to bioimmobilize environmental lead (Pb). Nevertheless, the underlying changes of Pb2+ biotoxicity in PSM during MIPP process were rarely discussed. The current study explored the Pb2+ immobili-zation and metabolic changes in PSM Penicillium oxalicum postexposure to Pb2+ and/or tricalcium phosphate (TCP). TCP addition significantly increased soluble P concentrations, accelerated extracellular Pb mineralization, and improved antioxidative enzyme activities in P. oxalicum during MIPP process. Secondary Pb2+ biomineral-ization products were measured as hydroxypyromorphite [Pb-10(PO4)(6)(OH)(2)]. Using untargeted metabolomic and transcriptomics, we found that Pb2+ exposure stimulated the membrane integrity deterioration and nucleotide metabolism obstruction of P. oxalicum. Correspondingly, P. oxalicum could produce higher levels of gamma- aminobutyric acid (GABA) to enhance the adaptive cellular machineries under Pb2+ stress. While the MIPP process improved extracellular Pb2+ mineralization, consequently alleviating the nucleotide metabolism inhi-bition and membrane deterioration. Multi-omics results suggested that GABA degradation pathway was stimu-lated for arginine biosynthesis and TCA cycle after Pb2+ mineralization. These results provided new biomolecular information underlying the Pb2+ exposure biotoxicities to microorganisms in MIPP before the application of this approach in environmental Pb2+ remediation.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据