期刊
JOURNAL OF EXPERIMENTAL & CLINICAL CANCER RESEARCH
卷 41, 期 1, 页码 -出版社
BMC
DOI: 10.1186/s13046-022-02458-3
关键词
LncMMPA; Hepatocellular carcinoma; Tumor associated macrophage; Metabolic reprogramming
类别
资金
- National Natural Science Foundation of China [82172799, 82073208, 82103521]
This study found that tumor-associated macrophages enhance aerobic glycolysis and proliferation in hepatocellular carcinoma cells by transmitting a long non-coding RNA called lncMMPA through exosomes. Furthermore, lncMMPA increases the proliferation of HCC cells by interacting with miR-548s. Clinically, lncMMPA expression is associated with glycolysis in TAMs and reduced survival of HCC patients.
Background Tumor-associated macrophages (TAMs), which form a large part of the tumor microenvironment, are normally regulated by metabolic reprogramming. However, the potential mechanisms of the immune-metabolism interaction between hepatocellular carcinoma (HCC) cells and TAMs remain unclear. Methods The candidate long non-coding RNAs (lncRNAs) were screened by Smart-seq based scRNA-seq method and then validated by qPCR. Immunostaining analysis was done to examine the levels of markers for TAMs and glycolysis. Exosomes from primary TAMs of human HCC tissues were isolated by centrifugation, and their internalization with lncRNAs was confirmed by immunofluorescence. The underlying mechanism of TAMs-derived exosomal lncRNA to HCC was confirmed by luciferase reporter assay and RNA immunoprecipitation. Metabolism regulation was evaluated through glucose consumption, lactate productions and extracellular acidification rates (ECARs). Mouse xenograft models were used to elucidate the in vivo effect of candidate lncRNAs on tumor growth. Results TAMs augment the aerobic glycolysis in HCC cells and their proliferation by the extracellular exosome transmission of a myeloid-derived lncRNA, M2 macrophage polarization associated lncRNA (lncMMPA). Mechanistically, lncMMPA not only could polarize M2 macrophage, but also could act as an microRNA sponge to interact with miR-548 s and increase the mRNA level of ALDH1A3, then further promote glucose metabolism and cell proliferation in HCC. Moreover, lncMMPA increased HCC cell multiplication through interacting with miR-548 s in vivo. Clinically, lncMMPA expression associates with glycolysis in TAMs and reduced survival of HCC patients. Conclusion LncMMPA plays an important role in regulating HCC malignancy and metabolic reprogramming of miR-548 s/ALDH1A3 pathway.
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