期刊
出版社
TAYLOR & FRANCIS INC
DOI: 10.1080/03601234.2022.2115780
关键词
Glyphosate; toxicity; development; transcriptomic; zebrafish
资金
- National Council for Science and Technology (CONACYT) [CB2016-288306, CVU695082]
This study aimed to evaluate the effects of glyphosate at environmental concentrations on zebrafish embryos. The results showed that exposure to high concentrations of glyphosate may lead to a decrease in heart rate in zebrafish embryos. In addition, transcriptomic analysis revealed subtle changes in gene expression profiles, including genes related to oocyte maturation, metabolic processes, histone deacetylation, and nervous system development.
Glyphosate [N-(phosphonomethyl)glycine] is one of the most popular herbicides worldwide. Globally, the use of glyphosate is increasing, and its residues have been found in drinking water and food products. The data regarding the possible toxic effects of this herbicide are controversial. Therefore, the aim of this study was to evaluate the effects of glyphosate at environmental concentrations in zebrafish (Danio rerio) embryos. Embryos were exposed to 0, 1, 100, and 1,000 mu g/L glyphosate for 96 h, and mortality, heart rate, and hatching rate were evaluated. After the experiment, RNA was extracted from the embryos for transcriptional analysis. No mortality was recorded, and exposure to 100 mu g/L and 1,000 mu g/L of glyphosate resulted in lower heart rates at 48 h. In addition, RNA-seq analysis revealed that glyphosate exposure induced subtle changes in gene transcription profiles. We found 30 differentially expressed genes; however, the highest glyphosate concentration (1,000 mu g/L) induced the greatest number of differentially expressed genes involved in oocyte maturation, metabolic processes, histone deacetylation, and nervous system development.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据