期刊
JOURNAL OF ENDOCRINOLOGY
卷 254, 期 3, 页码 199-211出版社
BIOSCIENTIFICA LTD
DOI: 10.1530/JOE-22-0043
关键词
Leydig cells; luteinizing hormone; steroidogenesis; mitochondria; cholesterol
资金
- National Institutes of Health
- [DK110059]
This study uncovered that murine Leydig cells remain responsive to exogenous cholesterol delivery by lipoproteins in the presence of serum, highlighting the importance of Rho-GTPase signaling pathways in the regulation of steroid biosynthesis. These findings challenge the long-held view of serum interference and underscore the need for exogenous lipoproteins for studying cholesterol trafficking and physiological signaling in vitro. The LH-responsive MA-10(Slip) clones derived in this study present a reformed platform for studying mammalian steroidogenesis.
MA-10 cells, established 4 decades ago from a murine Leydig cell tumor, has served as a key model system for studying steroidogenesis. Despite a precipitous loss in their innate ability to respond to luteinizing hormone (LH), the use of a cell-permeable cAMP analog for induction ensured their continued use. In parallel, a paradigm that serum-free conditions are essential for trophic steroidogenic stimulation was rationalized. Through the selection of LH-responsive single-cell MA-10(Slip) clones, we uncovered that Leydig cells remain responsive in the presence of serum in vitro and that exogenous cholesterol delivery by lipoproteins provided a significantly elevated steroid biosynthetic response (> 2-fold). In scrutinizing the underlying regulation, systems biology of the MA-10 cell proteome identified multiple Rho-GTPase signaling pathways as highly enriched. Testing Rho function in steroidogenesis revealed that its modulation can negate the specific elevation in steroid biosynthesis observed in the presence of lipoproteins/serum. This signaling modality primarily linked to the regulation of endocytic traffic is evident only in the presence of exogenous cholesterol. Inhibiting Rho function in vivo also decreased hCG-induced testosterone production in mice. Collectively, our findings dispel a long-held view that the use of serum could confound or interfere with trophic stimulation and underscore the need for exogenous lipoproteins when dissecting physiological signaling and cholesterol trafficking for steroid biosynthesis in vitro. The LH-responsive MA-10(Slip) clones derived in this study present a reformed platform enabling biomimicry to study the cellular and molecular basis of mammalian steroidogenesis.
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