期刊
CARBOHYDRATE POLYMERS
卷 117, 期 -, 页码 115-122出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.carbpol.2014.09.039
关键词
Fructo-oligosaccharides; Immunomodulation; Inulin; Mitogenicity; Onion; Phagocytosis
资金
- Council of Scientific and Industrial Research (CSIR), New Delhi [MLP-0101]
Onion (Allium cepa) is an immune-boosting food rich in fructans. The major aim of this study is to characterize and investigate the immunomodulatory properties of onion fructo-oligosaccharides (FOS). FOS was isolated from onion bulbs by hot 80% ethanol extraction (yield: similar to 4.5 g/100 g fw) followed by gel permeation chromatography. NMR of onion FOS revealed unusual p-D-Glc terminal residue at the non-reducing end. TLC and ESI-MS analyses showed that onion FOS ranged from trisaccharides to hexasaccharides. Onion FOS (50 mu g/mL) significantly increased (similar to 3-fold) the proliferation of mouse splenocytes/thymocytes vs. control. Further, onion FOS enhanced (similar to 2.5-fold) the production of nitric oxide by peritoneal exudates cells (PECs) from Wistar rats; intracellular free radicals production and phagocytic activity of isolated murine PECs were also augmented. Our structural and in vitro results indicate that onion FOS comprising of tri- to hexasaccharide units belongs to inulin-type fructans, and possess immunostimulatory activities towards murine lymphocytes and macrophages. (C) 2014 Elsevier Ltd. All rights reserved.
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