期刊
JOURNAL OF INORGANIC BIOCHEMISTRY
卷 156, 期 -, 页码 133-144出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.jinorgbio.2015.12.018
关键词
Antitumor properties; Gold(I); Silver(I); Copper(I); Amino acid derivatives; Apoptosis
资金
- Ministerio de Economia y Competitividad-FEDER [CTQ2013-48635-C2-1-P]
- DGA-FSE [E77]
Gold(I), gold(III), silver(I) and copper(I) complexes with modified amino acid esters and phosphine ligands have been prepared in order to test their cytotoxic activity. Two different phosphine fragments, PPh3 and PPh(2)py (py = pyridine), have been used. The amino acid esters have been modified by introducing an aromatic amine as pyridine that coordinates metal fragments through the nitrogen atom, giving complexes of the type [M(L)(PR3)](+) or [AuCl3(L)] (L = L-valine-N-(4-pyridylcarbonyl) methyl ester (L1), L-alanine-N-(4-pyridylcarbonyl) methyl ester (12), L-phenylalanine-N-(4-pyridylcarbonyl) methyl-ester) (13); M = Au(I), Ag(I), Cu(I), PR3 = PPh3, PPh(2)py). The in vitro cytotoxic activity of metal complexes was tested against four tumor human cell lines and one tumor mouse cell line. A metabolic activity test (3-(4,5-dimethyl-thiazol-2-yl)-2,5-diphenyl tetrazolium bromide, MTT) was used and IC50 values were compared with those obtained for cisplatin. Several complexes displayed significant cytotoxic activities. In order to determine whether antiproliferation and cell death are associated with apoptosis, NIH-3T3 cells were exposed to five selected complexes (Annexin V+ FITC, PI) and analyzed by flow cytometry. These experiments showed that the mechanism by which the complexes inhibit cell proliferation inducing cell death in NIH-3T3 cells is mainly apoptotic. (C) 2015 Elsevier Inc. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据