期刊
出版社
ELSEVIER
DOI: 10.1016/j.jchromb.2022.123473
关键词
Fatty acids; Method validation; Transesterification; Gas chromatography-ion trap mass; spectrometry
资金
- JIPMER Intramural Research Committee, Pondicherry
- Indian Council Medical Research, New Delhi
- [52/19/2020-BIO/BMS]
Fatty acids play a role in physiological functions and diseases. The optimized GC-IT/MS method allows for the simultaneous quantification of total fatty acids in plasma, providing important information for disease risk prediction, diagnosis, and prognosis.
Fatty acids (FAs) are associated with many physiological functions of tissues, and their alteration has been linked with tissue-specific or systemic diseases. The current situation warrants us to have a sensitive and specific method for analysis of total FAs simultaneously from the biological fluid so that the risk prediction, diagnosis or prognosis of the disease can be made effectively. Because of greater sensitivity and resolution, a method of gas chromatography-ion trap mass spectrometry (GC-IT/MS) has been optimized and validated to quantify simul-taneously 19 total FAs levels in plasma and compared with GC-triple quadrupole mass spectrometry. FAs have been transesterified by methanolic acetyl chloride to fatty acid methyl esters (FAMEs). A 65 min GC method separated all 19 FAMEs. The calibration curve had good linearity up to 313-922 mu M with a correlation coefficient between 0.9882 and 0.9998. The LODs and LOQs of FAMEs were in the range of 0.63 to 9.55 and 2.12 to 31.8 mu M, respectively. The method has recovery up to 144 %, stability at 4 degrees C for 48 h and one freeze-thaw cycle, and good intra-day and inter-day precision. The optimized method has been used to quantify plasma total FAs in type 2 diabetes mellitus patients with and without acute coronary syndrome. Though a significant difference has been found between IT/MS and triple quadrupole mass spectrometry, the GC-IT/MS can help to quantify total FAs in the clinical setting.
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