Superhydrophobic fluorinated microspheres for fluorous affinity chromatography

Fluorous affinity chromatography has received growing attention in separation and purification of fluoro compounds, but the wettability of the fluorinated stationary phases is seldom noticed. Here, we construct a series of micro-sized fluorine-containing microspheres by solvothermal precipitation polymerization. The fluorinated microspheres could be obtained with narrow size distribution at even high monomer loading of 15 wt%. Through alternating fluoro monomer, both the particle size and the wettability of the microsphere array could be tuned. Among them, the poly(divinylbenzene -dodecafluoroheptyl methacrylate), P(DVB-DFHMA), microsphere (6.1 mu m) arrays displays superhydrophobicity with 153.2 ?degrees water contact angle. The P(DVB-DFHMA) fluorinated microspheres (7.58% fluorine content) can be packed into steel-less columns as stationary phase for high-performance liquid chromatography. The retention mechanism of the fluorinated column is proven to be the specific fluorine-fluorine interaction. Compared to the commercial C18 silica column, the fluorinated column can completely separate fluorine-containing compounds under high water content mobile phase, including small fluoro molecules and fluoro macro-molecules, at much lower back pressure by fluorous affinity. (C) 2022 Elsevier B.V. All rights reserved.

Automated summary

This study describes the synthesis of micro-sized fluorinated microspheres and demonstrates their tunable particle size and wettability through alternating fluorine monomers. The resulting microspheres exhibit superhydrophobicity and can be utilized as a stationary phase for high-performance liquid chromatography.