期刊
JOURNAL OF INORGANIC BIOCHEMISTRY
卷 161, 期 -, 页码 115-121出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.jinorgbio.2016.05.010
关键词
Binding constant; Circular dichroism; Computational chemistry; DNA G-quadruplex, nickel zinc
资金
- Mahlke-Obermann Stiftung
- European Union [609431]
Novel nickel(II) (1) and zinc(II) (2) complexes of a Salen-like ligand, carrying a pyrimidine ring on the N,N' bridge, were synthesized and characterized. Their interaction with duplex and G-quadruplex DNA was investigated in aqueous solution through UV-visible absorption, circular dichroism and viscometry measurements. The results obtained point out that, while the zinc(II) complex does not interact with both duplex and G-quadruplex DNA, the nickel(II) complex 1 binds preferentially to G-quadruplex respect to duplex-DNA, with values of the DNA-binding constants, K-b, 2.6 x 10(5) M-1 and 3.5 x 10(4) M-1, respectively. Molecular dynamics simulations provided an atomic level model of the top-stacking binding occurring between 1 and hTelo (a 22-mer sequence oligonucleotide) in G-quadruplex conformation. (C) 2016 Elsevier Inc. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据