4.5 Article

Impaired repair properties of endothelial colony-forming cells in patients with granulomatosis with polyangiitis

期刊

JOURNAL OF CELLULAR AND MOLECULAR MEDICINE
卷 26, 期 19, 页码 5044-5053

出版社

WILEY
DOI: 10.1111/jcmm.17531

关键词

anti-neutrophil cytoplasmic antibody-associated vasculitis; endothelial colony-forming cells; endothelial progenitor cells; granulomatosis with polyangiitis

资金

  1. Coordination for the Improvement of Higher Education Personnel-Brazil (Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior, CAPES) [001]
  2. Sao Paulo Research Foundation (Fundacao de Amparo a Pesquisa do Estado de Sao Paulo, FAPESP) [2014/00984-3]

向作者/读者索取更多资源

This study found that ECFCs from patients with PR3-positive GPA in remission demonstrated early losses of tube formation and reduced migration capacity, suggesting impairment of endothelial function.
In patients with ANCA-associated vasculitis, interactions between neutrophils and endothelial cells cause endothelial damage and imbalance. Endothelial colony-forming cells (ECFCs) represent a cellular population of the endothelial lineage with proliferative capacity and vasoreparative properties. This study aimed to evaluate the angiogenic capacity of ECFCs of patients with granulomatosis with polyangiitis (GPA). The ECFCs of 13 patients with PR3-positive GPA and 14 healthy controls were isolated and characterized using fluorescence-activated cell sorting, capillary tube formation measurement, scratching assays and migration assays with and without plasma stimulation. Furthermore, three patients with active disease underwent post-treatment recollection of ECFCs for longitudinal evaluation. The ECFCs from the patients and controls showed similar capillary structure formation. However, the ECFCs from the patients with inactive GPA exhibited early losses of angiogenic capacity. Impairments in the migration capacities of the ECFCs were also observed in patients with GPA and controls (12th h, p = 0.05). Incubation of ECFCs from patients with GPA in remission with plasma from healthy controls significantly decreased migration capacity (p = 0.0001). Longitudinal analysis revealed that treatment significantly lowered ECFC migration rates. This study revealed that ECFCs from the patients with PR3-positive GPA in remission demonstrated early losses of tube formation and reduced migration capacity compared to those of the healthy controls, suggesting impairment of endothelial function.

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