期刊
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A
卷 111, 期 1, 页码 45-59出版社
WILEY
DOI: 10.1002/jbm.a.37444
关键词
cell-material interaction; dental implants; molecular biocompatibility; mTOR signaling; osseointegration
This study investigated the effect of sandblasted acid-etched (SAE) titanium implants on the biological responses of human osteoblasts using proteomic analysis. The results showed that SAE-Ti increased mineralization and expression of specific genes in osteoblasts, thereby enhancing osseointegration.
Titanium is widely used in bone prostheses due to its excellent biocompatibility and osseointegration capacity. To understand the effect of sandblasted acid-etched (SAE) Ti implants on the biological responses of human osteoblast (HOb), their proteomic profiles were analyzed using nLC-MS/MS. The cells were cultured with the implant materials, and 2544 distinct proteins were detected in samples taken after 1, 3, and 7 days. Comparative analyses of proteomic data were performed using Perseus software. The expression of proteins related to EIF2, mTOR, insulin-secretion and IGF pathways showed marked differences in cells grown with SAE-Ti in comparison with cells cultured without Ti. Moreover, the proteomic profiles obtained with SAE-Ti were compared over time. The affected proteins were related to adhesion, immunity, oxidative stress, coagulation, angiogenesis, osteogenesis, and extracellular matrix formation functions. The proliferation, mineralization and osteogenic gene expression in HObs cultured with SAE-Ti were characterized in vitro. The results showed that the osteoblasts exposed to this material increase their mineralization rate and expression of COLI, RUNX2, SP7, CTNNB1, CAD13, IGF2, MAPK2, and mTOR. Overall, the observed proteomic profiles can explain the SAE-Ti osteogenic properties, widening our knowledge of key signaling pathways taking part in the early stages of the osseointegration process in this type of implantations.
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