期刊
JOURNAL OF BIOLOGICAL CHEMISTRY
卷 298, 期 11, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.jbc.2022.102510
关键词
-
资金
- Council of Scientific and Industrial Research (CSIR) NCP Project [MLP-136]
- Department of Science and Technology [CRG/2021/000421]
- CSIR fellowships
- UGC fellowships
This study reports two members of the ChaC family of gamma-glutamyl cyclotransferases in Leishmania, showing their roles in GSH degradation and cell growth.
The ChaC family of gamma-glutamyl cyclotransferases is conserved throughout all Kingdoms and catalyzes the degradation of GSH. So far, the ChaC family proteins in trypanosomal parasites are missing in the literature. Here, we report two members of the ChaC family of gamma-glutamyl cyclotransferases (LmChaC2a and LmChaC2b) in the unicellular pathogen Leishmania. Activity measurements suggest that these proteins catalyze degradation of GSH but no other gamma-glutamyl peptides. Recombinant LmChaC2a protein shows 17-fold lower catalytic efficiency (kcat 0.9 s-1) than LmChaC2b (kcat 15 s-1), although they showed comparable Km values ( 1.75 mM for LmChaC2a and 2.0 mM for LmChaC2b) toward GSH. qRT-PCR and Western blot analyses suggest that the LmChaC2a protein was found to be constitutively expressed, whereas LmChaC2b was regulated by sulfur stress. To investigate its precise physiological function in Leishmania, we generated overexpressed, knockout, and com-plement cell lines. Flow cytometric analyses show the presence of a higher intracellular GSH concentration and lower intra-cellular ROS level, indicative of a more reductive environment in null mutants. We found LmChaC2-expressing cells grow in GSH-containing sulfur-limited media, while the null mutants failed to grow, suggesting that LmChaC2 is crucial for cell growth with GSH as the only sulfur source. Null mutants, although reach the stationary phase rapidly, display impaired long-term survival, indicating that LmChaC2-mediated GSH degradation is necessary for prolonged survival. In vivo studies suggest that LmChaC2-dependent controlled GSH degradation promotes chronic infection by the parasite. Altogether, these data indicate that LmChaC2 plays an important role in GSH homeostasis in Leishmania.
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