期刊
JOURNAL OF BIOLOGICAL CHEMISTRY
卷 298, 期 12, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.jbc.2022.102636
关键词
-
资金
- National Institutes of Health [NEI R01EY012224]
This study investigates the role of cAMP-dependent GRK phosphorylation in cone photoreceptor recovery. Experiment results in zebrafish show that elevated cAMP levels significantly decrease the recovery of cone photoresponse, mediated by Grk7a instead of Grk1b. Using a cone-specific dominant negative PKA transgene, it is demonstrated for the first time that PKA is required for the phosphorylation of Grk7a in vivo. Furthermore, immunoblot analyses reveal that cone-expressed Grk1 does not undergo cAMP-dependent phosphorylation in vivo.
In the vertebrate retina, phosphorylation of photoactivated visual pigments in rods and cones by G protein-coupled receptor kinases (GRKs) is essential for sustained visual function. Previous in vitro analysis demonstrated that GRK1 and GRK7 are phosphorylated by PKA, resulting in a reduced capacity to phosphorylate rhodopsin. In vivo observations revealed that GRK phosphorylation occurs in the dark and is cAMP dependent. In many vertebrates, including humans and zebrafish, GRK1 is expressed in both rods and cones while GRK7 is expressed only in cones. However, mice express only GRK1 in both rods and cones and lack GRK7. We recently generated a mutation in Grk1 that deletes the phosphorylation site, Ser21. This mutant demonstrated delayed dark adaptation in mouse rods but not in cones in vivo, suggesting GRK1 may serve a different role depending upon the photoreceptor cell type in which it is expressed. Here, zebrafish were selected to evaluate the role of cAMP-dependent GRK phosphorylation in cone photoreceptor recovery. Electroretinogram analyses of larvae treated with forskolin show that elevated intracellular cAMP significantly decreases recovery of the cone photoresponse, which is mediated by Grk7a rather than Grk1b. Using a cone-specific dominant negative PKA transgene, we show for the first time that PKA is required for Grk7a phosphorylation in vivo. Lastly, immunoblot analyses of rod grk1a-/- and cone grk1b-/- zebrafish and Nrl-/- mouse show that coneexpressed Grk1 does not undergo cAMP-dependent phosphorylation in vivo. These results provide a better understanding of the function of Grk phosphorylation relative to cone adaptation and recovery.
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