期刊
JOURNAL OF BASIC MICROBIOLOGY
卷 63, 期 1, 页码 26-38出版社
WILEY
DOI: 10.1002/jobm.202200530
关键词
Alternaria alternata; antifungal activity; biocontrol; lichenysin
类别
This study identified the antifungal metabolites produced by Bacillus altitudinis Q7 as lichenysin and investigated its antifungal activity and action mechanism. Lichenysin inhibited Alternaria alternata by altering the permeability of the cell membrane, leading to the release of proteins and nucleic acids from the cytoplasm. The stability of lichenysin in extreme environments suggests its potential as a biological control agent against A. alternata.
This study identified the antifungal metabolites produced by Bacillus altitudinis Q7 against Alternaria alternata and investigated the antifungal activity and antifungal action. Lipopeptide, the important secondary metabolites were identified by Fourier transform infrared (FTIR) and liquid chromatography-mass spectrometry as lichenysin. The antifungal activity of lipopeptide on A. alternata was determined by microdilution technique, and its minimum inhibitory concentration was 1.2 mg/ml. Stability test showed that lipopeptide had excellent temperature and pH resistance. To investigate whether lichenysin acted on the cell membrane and changed its permeability, the ultra-violet absorption of protein and nucleic acid were measured using a colorimetric method. The antifungal metabolites produced by B. altitudinis Q7 was lichenysin, which showed stable antifungal activity in the extreme environments. Lichenysin could inhibit A. alternata by altering the permeability of cell membrane, leading to the outflow of proteins and nucleic acids from the cytoplasm. This research suggests the lipopeptide from B. altitudinis Q7 is a potential biological control agent against A. alternata.
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