4.7 Article

New Mass Spectrometric Approach to Quantify the Major Isoallergens of the Apple Allergen Mal d 1

期刊

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
卷 70, 期 37, 页码 11813-11822

出版社

AMER CHEMICAL SOC
DOI: 10.1021/acs.jafc.2c03745

关键词

bottom-up proteomics; marker peptides; isotope dilution analyses; protein extraction; apple allergy

资金

  1. German Research Foundation (DFG) [3811/1-1]
  2. Ministry of Science, Research and the Arts Baden-Wurttemberg
  3. Dr. Leni Schoninger Foundation
  4. funds of the chemical industry, Germany (FCI)

向作者/读者索取更多资源

Patients with birch pollinosis often have adverse reactions to fresh apples due to the similarity of allergens in birch and apples. A proteomics approach was developed to quantify the allergen Mal d 1 in apples. Marker peptides for different isoallergens of Mal d 1 were identified, and the isoallergen-specific content in different apple varieties was quantified.
Patients who suffer from birch pollinosis often develop adverse reactions to the consumption of fresh apples due to the structural similarity of the allergens Bet v 1 and Mal d 1 from birch and apples, respectively. A different allergenic potential for Mal d 1 isoallergens is postulated, but approaches to quantify the Mal d 1 isoallergen-specific are missing. Therefore, a bottom-up proteomics approach was developed to quantify Mal d 1 by stable isotope dilution and microHPLC-QTOF analyses. Marker peptides for individual isoallergens (Mal d 1.01-Mal d 1.03 and Mal d 1.06), combinations thereof (Mal d 1.01 + 1.02, Mal d 1.02 + 1.06, and Mal d 1.04 + 1.05), and two global marker peptides, comprising Mal d 1.01 + 1.02 + 1.04 + 1.05 and Mal d 1.03 + 1.06 + 1.07 + 1.08 + 1.09, were identified. By the use of an extraction standard (r-Mal d 1_mut), an optimized protocol for extraction and tryptic digestion of apple proteins was developed, and the variety-specific extraction efficiency was monitored for the flesh and peel of apples. The Mal d 1 contents in flesh and peel of five commercial apple breeds and four apple varieties from orchard meadows were quantified isoallergen-specific.

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