期刊
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
卷 70, 期 41, 页码 13349-13357出版社
AMER CHEMICAL SOC
DOI: 10.1021/acs.jafc.2c04455
关键词
arabinoxylan; diferulic acid; feruloyl esterase; carbohydrate-binding module 48
资金
- China Scholarship Council
- Technical University of Denmark
A new enzyme activity has been discovered that can directly release diferulic acids from corn bran, providing new insights and opportunities for solving the problem of recalcitrant arabinoxylans.
Corn bran is an abundant coprocessing stream of corn-starch processing, rich in highly substituted, diferuloyl-crosslinked glucurono-arabinoxylan. The diferuloyl cross-links make the glucurono-arabinoxylan recalcitrant to enzymatic conversion and constitute a hindrance for designing selective enzymatic upgrading of corn glucurono-arabinoxylan. Here, we show that two bacterial feruloyl esterases, wtsFae1A and wtsFae1B, each having a carbohydrate-binding module of family 48, are capable of cleaving the ester bonds of the cross-linkages and releasing 5-5 ', 8-5 ', 8-5 ' benzofuran, and 8-O-4 ' diferulate from soluble and insoluble corn bran glucurono-arabinoxylan. All four diferulic acids were released at similar efficiency, indicating nondiscriminatory enzymatic selectivity for the esterified dimer linkages, the only exception being that wtsFae1B had a surprisingly high propensity for releasing the dimers, especially 8-5 ' benzofuran diferulate, indicating a potential, unique catalytic selectivity. The data provide evidence of direct enzymatic release of diferulic acids from corn bran by newly discovered feruloyl esterases, i.e., a new enzyme activity. The findings yield new insight and create new opportunities for enzymatic opening of diferuloyl cross-linkages to pave the way for upgrading of recalcitrant arabinoxylans.
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