期刊
CARBOHYDRATE POLYMERS
卷 133, 期 -, 页码 104-109出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.carbpol.2015.07.014
关键词
Ganoderma lucidum; Exopolysaccharide; Monosaccharide composition; PGM; PGI
资金
- National Natural Science Foundation of China [31271918]
- National High Technology Research and Development Program of China 863 Program [2012AA021505]
- Fundamental Research Funds for the Central Universities [JUSRP51319B]
- Open Project Program of Key Laboratory of Carbohydrate Chemistry and Biotechnology [KLCCB-KF201205]
We investigated the relationship between monosaccharide composition of Ganoderma lucidum exopolysaccharide (EPS) and activities of EPS synthesis enzymes under various culture temperatures and initial pH values. The mole percentages of three major EPS monosaccharides, glucose, galactose and mannose, varied depending on culture conditions and the resulting EPS displayed differing anti-tumor activities. In nine tested enzymes, higher enzyme activities were correlated with higher temperature and lower initial pH. Altered mole percentages of galactose and mannose under various culture conditions were associated with activities of alpha-phosphoglucomutase (PGM) and phosphoglucose isomerase (PGI), respectively, and that of mannose was also associated with phosphomannose isomerase (PMI) activity only under various pH. Our findings suggest that mole percentages of G. lucidum EPS monosaccharides can be manipulated by changes of culture conditions that affect enzyme activities, and that novel fermentation strategies based on this approach may enhance production and biological activity of EPS. (C) 2015 Elsevier Ltd. All rights reserved.
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