期刊
JOURNAL OF INFECTIOUS DISEASES
卷 214, 期 6, 页码 925-934出版社
OXFORD UNIV PRESS INC
DOI: 10.1093/infdis/jiw201
关键词
S. oralis; C. albicans; calpain; synergy; E-cadherin; oral mucosa
资金
- US Public Health Service through the National Institute of Dental and Craniofacial Research [RO1 DE013986]
Streptococcus oralis forms robust mucosal biofilms with Candida albicans that have increased pathogenic potential. In this study, using oral epithelial cultures, organotypic oral mucosal constructs, and a mouse model of oral infection, we demonstrated that S. oralis augmented C. albicans invasion through epithelial junctions. C. albicans and S. oralis decreased epithelial E-cadherin levels by synergistically increasing A mu-calpain, a proteolytic enzyme that targets E-cadherin. In the mouse coinfection model this was accompanied by increased fungal kidney dissemination. Coinfection with a secreted aspartyl protease (sap) mutant sap2456 and S. oralis increased mu-calpain and triggered mucosal invasion and systemic dissemination, suggesting that fungal protease activity is not required for invasion during coinfection. We conclude that C. albicans and S. oralis synergize to activate host enzymes that cleave epithelial junction proteins and increase fungal invasion.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据