Moraxella nasovis sp. nov., isolated from a sheep with respiratory disease

A novel Gram- stain- negative, aerobic, coccus- shaped bacteria, designated ZY201115T, was isolated from the nasal cavity of a sheep with respiratory disease in Yunnan Province, south- west China, and its taxonomic affiliation was studied by applying a polyphasic approach. The strain grew at 18-41 degrees C (optimum, 37 degrees C), at pH 6.0-9.0 (optimum, pH 8.0) and in 0.5-3.0% (w/v) NaCl (optimum, 1.0 % NaCl). Phylogenetic analysis based on 16S rRNA gene sequences showed that the strain is affiliated to the genus Moraxella with highest similarity to Moraxella bovis ATCC 10900T (96.6 %). Phylogenomic analysis based on 811 singlecopy genes also indicated that the strain represents a novel species in the genus Moraxella and formed a deep and separated clade with Moraxella caviae NCTC 10293T. The highest genomic orthologous average nucleotide identity and digital DNA-DNA hybridization values between the strain and the type strains in the genus Moraxella were 73.7% (M. caviae NCTC 10293T) and 25.3% (Moraxella osloensis CCUG 350T), respectively. The G+C content of the complete genome sequence was 42.1 mol%. The predominant fatty acids (>5 %) were C18:1 w9c, C17:1 w8c, C12:03OH and summed feature 3 (C16:1 w7c and/or C16:1 w6c). The major polar lipids were phosphatidylglycerol, cardiolipin, monolysocardiolipin, phosphatidylethanolamine and hemibismonoacylglycerophosphate. The major respiratory quinone was CoQ- 8. On the basis of the results of phylogenetic, phenotypic and chemotaxonomic characterizations, strain ZY201115T clearly represents a novel species of the genus Moraxella, for which the name Moraxella nasovis sp. nov. is proposed. The type strain is ZY201115T (=CCTCC AB 2021473T=CCUG 75922T).

Automated summary

A novel Gram-stain-negative bacteria, Moraxella nasovis sp. nov., was isolated from the nasal cavity of a sheep with respiratory disease in Yunnan Province, China. It showed similarity to other species in the genus Moraxella, but also formed a distinct clade. This study provides valuable taxonomic information on this newly discovered species.