Limibaculum sediminis sp. nov., isolated from mangrove sediment

A Gram-stain-negative, cream-coloured, aerobic, motile and ovoid- to rod-shaped bacterium, designated as FT325T, was isolated from mangrove sediment collected in Shenzhen, PR China. The taxonomic position of strain FT325T was established by phylogenetic, physiological, biochemical and chemotaxonomic analyses. Strain FT325T grew optimally at 37-40 degrees C and pH 6.0 in the presence of 0 % (w/v) NaCl. Results of 16S rRNA gene sequence analysis showed that strain FT325T was most similarly related to Limibaculum halophilum CAU 1123T (96.2 %), Phaeovulum vinaykumarii DSM 18714T (93.9%) and Amaricoccus solimangrovi HB 172011T (93.7%). The major fatty acids (>10%) were C 18:1.7c (60.0%) and 11-methyl C 18: 1.7c (16.7%). The sole respiratory quinone was Q-10. The polar lipids were phosphatidylglycerol, one unidentified glycolipid, three unidentified aminolipids and three unidentified phospholipids. Its estimated genome size was 4318768 bp and the genomic DNA G+C content was 69.6mol%. Based on its distinct phenotypic, chemotaxonomic and phylogenetic characteristics, strain FT325T represents a novel species of the genus Limibaculum, for which the name Limibaculum sediminis sp. nov. is proposed (=MCCC 1K07397T=KCTC 92313T).

Automated summary

In this study, a cream-coloured Gram-stain-negative bacterium, designated as Limibaculum sediminis sp. nov., was isolated from mangrove sediment in Shenzhen, PR China. The taxonomic position and characteristics of the bacterium were determined using various phylogenetic, physiological, biochemical and chemotaxonomic analyses.