期刊
INTERNATIONAL JOURNAL OF PHARMACEUTICS
卷 625, 期 -, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.ijpharm.2022.122062
关键词
Cichorium intybus; Breast cancer; Silver nanoparticles; Callus; Selective cytotoxicity; Anti-bacterial
资金
- Alzahra University, Tehran, Iran [500-3-589]
This study synthesized silver nanoparticles using C. intybus leaf-derived callus extract and evaluated their antibacterial, antioxidant, and anticancer activities. The results showed that these silver nanoparticles exhibited significant cytotoxicity against breast cancer cells and inhibitory effects on cell proliferation. Additionally, they demonstrated strong antibacterial and antioxidant activities.
Cichorium intybus, commonly called chicory, has been widely used as a coffee substitute. It display a wide range of natural compounds and medicinally uses in treatment of gastrointestinal disorders. This study synthesized silver nanoparticles (Ci-AgNPs) using C. intybus leaf-derived callus extract to evaluate phytochemical content, antibacterial, antioxidant and anti-proliferative activities against human breast cancer cells (MDA-MB231). The optimal shape, size and stability of Ci-AgNPs was confirmed using UV-visible spectrophotometry, FESEM, EDX, XRD, DLS, Zeta potential, FTIR and sp-ICP-MS studies. The antibacterial activity of Ci-AgNPs was assessed using disk diffusion method against Staphylococcus aureus and Escherichia coli, and they displayed distinct zones of inhibition. Colorimetric phytochemical analysis of Ci-AgNPs revealed their higher total phenolic (TP) and total flavonoid (TF) content. Ci-AgNPs also indicated a high level of antioxidant activity using FRAP and DPPH assays. The Ci-AgNPs were investigated for their anticancer activities on the cancerous MDA-MB231 cells viability and apoptosis using MTT and flow cytometry, respectively. Ci-AgNPs showed dose dependent cytotoxicity against MDA-MB231 cells with IC50 value of 187.6 mu g/mL at 48 h through induction of apoptosis. The biocompatibility test showed that Ci-AgNPs induced neglectable cytotoxicity (lower than 3 %) toward human erythrocytes. This is the first study that reports the bio-callus mediated synthesis of silver nanoparticle using C. intybus callus extract which provided a promising anticancer activity against human breast cancer MDA-MB231 cells and therefore could be used as an alternative and interesting benign strategy for biosynthesis of silver nanoparticles useful in cancer therapy.
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