期刊
INTERNATIONAL JOURNAL OF NANOMEDICINE
卷 17, 期 -, 页码 3877-3892出版社
DOVE MEDICAL PRESS LTD
DOI: 10.2147/IJN.S374736
关键词
HDL; peptide-based HDL; atherosclerosis; drug delivery; natural plants; antiatherogenic therapy
资金
- Scientific and Technological Innovation Project, China Academy of Chinese Medical Sciences [CI2021A00112]
- Beijing Traditional Chinese Medicine Technology Development Foundation [JJ-2020-85]
- Fundamental Research Funds for the Central Public Welfare Research Institutes [YZ-202110]
This study utilized pHDL as a carrier to efficiently load lipophilic components from natural plants, resulting in improved bioavailability and reduction of atherosclerosis lesions. The findings suggest that pHDL-based drug delivery system has the potential to be applied in both imaging and therapy for atherosclerosis, while extending the application of bioactive chemicals from natural plants and facilitating the clinical translation of synthetic HDL therapies.
Purpose: Peptide-based high-density lipoprotein (pHDL) structurally and functionally resembles the natural HDL as anti -atherosclerosis (AS) therapies. Since pHDL contains a large hydrophobic core, this study aims to evaluate the potentials of pHDL as a hydrophobic drug carrier and the efficiency of drug-loaded pHDL in the control of AS. Methods: The pHDL encapsulation of hydrophobic components from natural plants, including curcumin (Cur) and tanshinone IIA (TanIIA), was achieved using one-step microfluidics. Then, morphological features and loading efficiencies of pHDL-Cur and pHDL-TanIIA were determined by TEM and high-performance liquid chromatography (HPLC), respectively. Taking the fluorescence advantage of Cur, localizations of loaded Cur in pHDL were investigated by fluorescence quenchers, and recruitments of Cur to AS plaques were assessed with ex vivo imaging. Based on anti-inflammatory properties of TanIIA, pHDL-TanIIA was accordingly developed to evaluate the anti-AS effects through examinations of plasma lipid parameters and pathological alterations of plaque-associated regions.Results: Both lipophilic Cur and TanIIA can be efficiently loaded into pHDL carriers. The resultant pHDL-Cur and pHDL-TanIIA inherit the homogeneous nano-disk structure of pHDL. By using pHDL-Cur, the encapsulated hydrophobics are tracked in the core of pHDL, and incorporations of Cur with pHDL vehicles greatly improve the bioavailability and association of Cur with AS plaques. Moreover, when loaded with TanIIA, which has established its role in anti-AS as an anti-inflammatory candidate, synergistic effects in reducing AS lesions and improving pathological alterations of main organs related to AS were achieved.Conclusion: The pHDL system could potentially be applied for both imaging and therapy in animal models of AS. Benefits of pHDL-based drug delivery will potentially extend the application scenarios of bioactive chemicals from natural plants which are underutilized due to features like low bioavailability and facilitate the clinical translation of synthetic HDL therapies in HDL-associated disorders, including but not limited to AS.
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