期刊
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
卷 23, 期 20, 页码 -出版社
MDPI
DOI: 10.3390/ijms232012555
关键词
nitrate; nitrite; processed meat; in vitro digestion; bioaccessibility; bioactive peptides; cellular toxicity
资金
- Italian POR-FESR 2014-2020 (Innovative Milk and Meat products for Consumer's Health-MiMe4Health)
- [986/2018]
- [PG/2018/632065]
Replacing synthetic nitrates/nitrites with natural additives can be a promising strategy to develop clean label salamis without negatively affecting their nutritional value.
Curing salts composed of mixtures of nitrates and nitrites are preservatives widely used in processed meats. Despite many desirable technological effects, their use in meat products has been linked to methemoglobinemia and the formation of nitrosamines. Therefore, an increasing anti-nitrite feeling has grown among meat consumers, who search for clean label products. In this view, the use of natural compounds as alternatives represents a challenge for the meat industry. Processing (including formulation and fermentation) induces chemical or physical changes of food matrix that can modify the bioaccessibility of nutrients and the formation of peptides, impacting on the real nutritional value of food. In this study we investigated the effect of nitrate/nitrite replacement with a combination of polyphenols, ascorbate, and nitrate-reducing microbial starter cultures on the bioaccessibility of fatty acids, the hydrolysis of proteins and the release of bioactive peptides after in vitro digestion. Moreover, digested salami formulations were investigated for their impacts on cell proliferation and genotoxicity in the human intestinal cellular model (HT-29 cell line). The results indicated that a replacement of synthetic nitrates/nitrites with natural additives can represent a promising strategy to develop innovative clean label salamis without negatively affecting their nutritional value.
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