4.7 Article

Mixture Effects of Tryptophan Intestinal Microbial Metabolites on Aryl Hydrocarbon Receptor Activity

期刊

出版社

MDPI
DOI: 10.3390/ijms231810825

关键词

aryl hydrocarbon receptor; tryptophan metabolites; indole derivatives; mimic mixtures; microbiome

资金

  1. Czech Science Foundation [20-00449S]
  2. Czech Ministry of Education, Youth and Sports projects CZ-OPENSCREEN [LM2018130]
  3. EATRIS-CZ [LM2018133]
  4. European Regional Development Fund project ENOCH [CZ. 02.1.01/0.0/0.0/16_019/0000868]
  5. European Union-Next Generation EU [LX22NPO5102]

向作者/读者索取更多资源

Aryl hydrocarbon receptor (AHR) plays important roles in intestinal physiology and is activated by microbial metabolites, with synergistic effects observed in combinations of low- or medium-efficacy agonists. High-efficacy agonists may show antagonist effects on AHR activation.
Aryl hydrocarbon receptor (AHR) plays pivotal roles in intestinal physiology and pathophysiology. Intestinal AHR is activated by numerous dietary, endogenous, and microbial ligands. Whereas the effects of individual compounds on AHR are mostly known, the effects of real physiological mixtures occurring in the intestine have not been studied. Using reporter gene assays and RT-PCR, we evaluated the combinatorial effects (3520 combinations) of 11 microbial catabolites of tryptophan (MICTs) on AHR. We robustly (n = 30) determined the potencies and relative efficacies of single MICTs. Synergistic effects of MICT binary mixtures were observed between low- or medium-efficacy agonists, in particular for combinations of indole-3-propionate and indole-3-lactate. Combinations comprising highly efficacious agonists such as indole-3-pyruvate displayed rather antagonist effects, caused by saturation of the assay response. These synergistic effects were confirmed by RT-PCR as CYP1A1 mRNA expression. We also tested mimic multicomponent and binary mixtures of MICTs, prepared based on the metabolomic analyses of human feces and colonoscopy aspirates, respectively. In this case, AHR responsiveness did not correlate with type of diet or health status, and the indole concentrations in the mixtures were determinative of gross AHR activity. Future systematic research on the synergistic activation of AHR by microbial metabolites and other ligands is needed.

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