Addition of Synthetic Biomaterials to Deproteinized Bovine Bone Mineral (DBBM) for Bone Augmentation-A Preclinical In Vivo Study

(1) Aim: To investigate the effect of synthetic bone substitutes, alpha-tricalcium phosphate (alpha-TCP) or bi-layered biphasic calcium-phosphate (BBCP) combined with deproteinized bovine bone mineral (DBBM), on bone formation. (2) Methods: Thirty critical size defects were randomly treated with the following five different treatment modalities: (1) negative control (NC, empty), (2) DBBM, (3) alpha-TCP + DBBM (1:1), (4) BBCP 3%HA/97%alpha-TCP + DBBM (1:1), and (5) BBCP 6%HA/94%alpha-TCP + DBBM (1:1). The samples, at four weeks post-surgery, were investigated by micro-CT and histological analysis. (3) Results: A similar level of new bone formation was demonstrated in the DBBM with alpha-TCP bone substitute groups when compared to the negative control by histomorphometry. DBBM alone showed significantly lower new bone area than the negative control (p = 0.0252). In contrast to DBBM, the micro-CT analysis revealed resorption of the alpha-TCP + DBBM, BBCP 3%HA/97%alpha-TCP + DBBM and BBCP 6%HA/94%alpha-TCP + DBBM, as evidenced by a decrease of material density (p = 0.0083, p = 0.0050 and p = 0.0191, respectively), without changing their volume. (4) Conclusions: New bone formation was evident in all defects augmented with biomaterials, proving the osteoconductive properties of the tested material combinations. There was little impact of the HA coating degree on alpha-TCP in bone augmentation potential and material resorption for four weeks when mixed with DBBM.

Automated summary

The aim of this study was to investigate the effect of synthetic bone substitutes in combination with deproteinized bovine bone mineral on bone formation. The results showed that the combination of alpha-TCP and DBBM resulted in similar levels of new bone formation compared to the negative control. Micro-CT analysis indicated resorption of the combined materials, but without any change in volume.