期刊
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
卷 23, 期 20, 页码 -出版社
MDPI
DOI: 10.3390/ijms232012323
关键词
protein arginine methyltransferase 7; SGC8158; DNA damage response; senescence; cell cycle
资金
- National Research Foundation of Korea (NRF)
- Korean government (MSIT) [2021R1A2C2013613, 2022R1A5A2021216]
- National Research Foundation of Korea [2021R1A2C2013613, 2022R1A5A2021216] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
PRMT7 is a critical regulator of cell growth and DNA damage response, and its inhibition can cause cell cycle arrest and disruption of DNA repair pathways. Combination with doxorubicin enhances its cytotoxic effect on cells.
Protein arginine methyltransferase 7 (PRMT7) regulates various cellular responses, including gene expression, cell migration, stress responses, and stemness. In this study, we investigated the biological role of PRMT7 in cell cycle progression and DNA damage response (DDR) by inhibiting PRMT7 activity with either SGC8158 treatment or its specific siRNA transfection. Suppression of PRMT7 caused cell cycle arrest at the G(1) phase, resulting from the stabilization and subsequent accumulation of p21 protein. In addition, PRMT7 activity is closely associated with DNA repair pathways, including both homologous recombination and non-homologous end-joining. Interestingly, SGC8158, in combination with doxorubicin, led to a synergistic increase in both DNA damage and cytotoxicity in MCF7 cells. Taken together, our data demonstrate that PRMT7 is a critical modulator of cell growth and DDR, indicating that it is a promising target for cancer treatment.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据