期刊
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
卷 23, 期 21, 页码 -出版社
MDPI
DOI: 10.3390/ijms232112824
关键词
H; pylori; vaccinology; cellular immunity; humoral immunity; immunoinformatic analysis; immunization; interleukins; immunoglobulins; stomach; histopathology
资金
- Deanship of Scientific Research, Imam Abdulrahman Bin Faisal University (IAU), Dammam [2018-219-Dent]
This study aimed to identify antigenic peptides in H. pylori and evaluate their effectiveness as potential vaccine candidates. The results showed that the SabA antigen was particularly effective in inducing an immune response against H. pylori in mice.
Helicobacter pylori (H. pylori) has been identified as a group-1 definite carcinogen. As of yet, there is no available vaccine for this microorganism. Our study aimed to identify antigenic peptides in H. pylori using an in silico proteomic approach, and to evaluate their effectiveness as potential vaccine candidates. Four different peptide sequences were prioritized using the reverse vaccinology, namely, CagA(1), CagA(2), VacA, and SabA. Peptides emulsified with Freunde's adjuvant were used to immunize BALB/C mice. Subcutaneously immunized mice were challenged by oral administration of H. pylori. IgG, IgA, IL4, and IL17 were detected in mice sera. Histopathology of the dissected stomach of vaccinated and control mice were assessed using H&E stain. IgG was significantly higher in mice vaccinated with SabA. IL-4 was significantly increased in CagA(1), CagA(2), VacA, and SabA vaccinated mice compared to the adjuvant group. Additionally, histopathological examination of gastric tissue showed a protective effect in the vaccinated groups compared to adjuvant and PBS groups. Our findings indicate a promising effect of the tested epitopes, particularly the SabA antigen, to induce an immune response against H. pylori.
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