期刊
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
卷 23, 期 18, 页码 -出版社
MDPI
DOI: 10.3390/ijms231810772
关键词
wheat quality; agronomic trait; mutation; breeding
资金
- National Natural Science Foundation of China [32072054, 31971939, 31901961]
- Science and Technology Department of Sichuan Province [2019YFH0066, 2020YFH0150]
In this study, two new Q alleles (Q(s1) and Q(c1)-N8) were obtained through ethyl methanesulfonate-induced mutagenesis. The Q(s1) allele did not significantly affect grain protein content (GPC) or other processing quality parameters, but it adversely affected grain yield (GY). In contrast, the Q(c1)-N8 allele positively affected GPC and GY. This research provides novel germplasm for wheat breeding and useful information for enhancing cereal crops.
Grain yield (GY) and grain protein content (GPC) are important traits for wheat breeding and production; however, they are usually negatively correlated. The Q gene is the most important domestication gene in cultivated wheat because it influences many traits, including GY and GPC. Allelic variations in the Q gene may positively affect both GY and GPC. Accordingly, we characterized two new Q alleles (Q(s1) and Q(c1)-N8) obtained through ethyl methanesulfonate-induced mutagenesis. Compared with the wild-type Q allele, Q(s1) contains a missense mutation in the sequence encoding the first AP(2) domain, whereas Q(c1)-N8 has two missense mutations: one in the sequence encoding the second AP(2) domain and the other in the microRNA172-binding site. The Q(s1) allele did not significantly affect GPC or other processing quality parameters, but it adversely affected GY by decreasing the thousand kernel weight and grain number per spike. In contrast, Q(c1)-N8 positively affected GPC and GY by increasing the thousand kernel weight and grain number per spike. Thus, we generated novel germplasm relevant for wheat breeding. A specific molecular marker was developed to facilitate the use of the Q(c1)-N8 allele in breeding. Furthermore, our findings provide useful new information for enhancing cereal crops via non-transgenic approaches.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据