期刊
CARBOHYDRATE POLYMERS
卷 115, 期 -, 页码 540-550出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.carbpol.2014.09.016
关键词
HILIC-MS/ELSD; Fingerprinting; Blockwise; De-methylesterification; Enzymology
资金
- European Community within a consortium PolyModE [KBBE-2007-3-3-07]
A gene encoding a putative pectinesterase from Bacillus licheniformis DSM13 was cloned and expressed in Escherichia coli. The resulting recombinant enzyme (BliPME) was purified and characterized as a pectin methylesterase. The enzyme showed maximum activity at pH 8.0 and 50 degrees C. BliPME is able to release up to 100% of the methylesters from lime pectin (DM 34-76 -> DM 0) and up to 73% of all methylesters from SBPs (DM 30-73 -> DM 14). BliPME efficiently de-methylesterifies lemon pectins and SBPs in a blockwise manner and is quite tolerant towards the acetyl groups present within the SBPs. Detailed analysis of the BliPME-modified pectins using HILIC-MSn and the classical calcium reactivity measurement showed that the enzyme generates pectins with low methylesterification (lime and SBP) and high acetyl content (SBP) while creating blocks of nonmethylesterified galacturonic acid residues. The high activity of BliPME towards highly methylesterified and acetylated pectins makes this novel esterase more efficient in removing methylesters from highly esterified beet pectin compared to other PMEs, e.g. Aspergillus niger PME. (C) 2014 Elsevier Ltd. All rights reserved.
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