4.7 Article

Isolation and Functional Analysis of VvWRKY28, a Vitis vinifera WRKY Transcription Factor Gene, with Functions in Tolerance to Cold and Salt Stress in Transgenic Arabidopsis thaliana

期刊

出版社

MDPI
DOI: 10.3390/ijms232113418

关键词

Beichun; VvWRKY28; abiotic stress; transgenic plant

资金

  1. Suqian Agricultural Science and Technology Independent Innovation Fund Project [SQCX202105]
  2. Suqian Modern Agriculture Leading Fund Project in 2021 (Demonstration and promotion of new grape varieties and supporting technologies for improving quality and efficiency)
  3. National Natural Science Foundation of China [32172521]

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This study isolated a new transcriptional regulator gene VvWRKY28 from grapes, which was found to be enriched in grape leaves and highly expressed under low temperature and high salt conditions. After transferring VvWRKY28 into Arabidopsis, it significantly improved the tolerance of Arabidopsis to low temperature and high salt, and also changed its physiological and biochemical indicators to cope with cold and high salt stimulation.
The grape (Vitis vinifera L.) not only has a long history of cultivation, but also has rich nutritional value and high economic value. However, grapes often face many threats in the growth process. For example, low temperature and salt stress restrict the growth status, yield, and geographical distribution of grapes. WRKY, as one of the largest transcription factor (TF) families in plants, participates in the response of plants to stress. VvWRKY28, a new zinc finger type transcriptional regulator gene, was isolated from Beichun (V. vinifera x V.amurensis) in this study. From the subcellular localization results, it can be concluded that VvWRKY28 was localized in the nucleus. The expression of VvWRKY28 was enriched in leaves (young and mature leaves), and cold and high salt conditions can induce high expression of VvWRKY28. After being transferred into Arabidopsis, VvWRKY28 greatly improved the tolerance of Arabidopsis to low temperature and high salt and also changed many physiological and biochemical indicators of transgenic Arabidopsis to cope with cold and high salt stimulation. The content of malondialdehyde (MDA) was decreased, but for chlorophyll and proline, their content increased, and the activities of superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT) were improved. In addition, under cold stress, binding with cis-acting elements promotes the expression of downstream genes related to cold stress (RAB18, COR15A, ERD10, PIF4, COR47, and ICS1). Moreover, it also plays an active role in regulating the expression of genes related to salt stress (NCED3, SnRK2.4, CAT2, SOD1, SOS2, and P5CS1) under salt stress. Therefore, these results provide evidence that VvWRKY28 may play a role in the process of plant cold and salt stress tolerance.

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