期刊
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
卷 23, 期 21, 页码 -出版社
MDPI
DOI: 10.3390/ijms232113276
关键词
thyroid cancer; disulfiram; cancer stem cells; c-Myc
资金
- National Science and Technology Council in Taiwan [111-2320-B-040-007-MY3]
Differentiated thyroid carcinomas (DTCs) are common types of endocrine malignancies. Cancer stem cells (CSCs) play a crucial role in the initiation, drug resistance, and metastasis of tumors. High intracellular aldehyde hydrogenase (ALDH) activity indicates the presence of CSCs in DTCs. Disulfiram (DSF), an ALDH inhibitor, has been found to target CSCs in various cancers when combined with copper. This study showed that DSF/copper can inhibit the growth of papillary and follicular DTCs by suppressing CSC activity. The molecular mechanisms involve the downregulation of BMI1 expression, a cell cycle-related protein, through inhibition of c-Myc- or E2F1-mediated pathways. DSF holds potential as a therapeutic agent for DTCs in the future.
Differentiated thyroid carcinomas (DTCs), which have papillary and follicular types, are common endocrine malignancies worldwide. Cancer stem cells (CSCs) are a particular type of cancer cells within bulk tumors involved in cancer initiation, drug resistance, and metastasis. Cells with high intracellular aldehyde hydrogenase (ALDH) activity are a population of CSCs in DTCs. Disulfiram (DSF), an ALDH inhibitor used for the treatment of alcoholism, reportedly targets CSCs in various cancers when combined with copper. This study reported for the first time that DSF/copper can inhibit the proliferation of papillary and follicular DTC lines. DSF/copper suppressed thyrosphere formation, indicating the inhibition of CSC activity. Molecular mechanisms of DSF/copper involved downregulating the expression of B lymphoma Mo-MLV insertion region 1 homolog (BMI1) and cell cycle-related proteins, including cyclin B2, cyclin-dependent kinase (CDK) 2, and CDK4, in a dose-dependent manner. BMI1 overexpression diminished the inhibitory effect of DSF/copper in the thyrosphere formation of DTC cells. BMI1 knockdown by RNA interference in DTC cells also suppressed the self-renewal capability. DSF/copper could inhibit the nuclear localization and transcriptional activity of c-Myc and the binding of E2F1 to the BMI1 promoter. Overexpression of c-Myc or E2F1 further abolished the inhibitory effect of DSF/copper on BMI1 expression, suggesting that the suppression of c-Myc and E2F1 by DSF/copper was involved in the downregulation of BMI1 expression. In conclusion, DSF/copper targets CSCs in DTCs by inhibiting c-Myc- or E2F1-mediated BMI1 expression. Therefore, DSF is a potential therapeutic agent for future therapy in DTCs.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据