4.7 Article

Binding of Glycerol to Human Galectin-7 Expands Stability and Modulates Its Functions

期刊

出版社

MDPI
DOI: 10.3390/ijms232012318

关键词

galectin-7; glycerol; stability; crystal structure; aquaporin-3

资金

  1. National Natural Science Foundation of China [82201916]
  2. Natural Science Foundation of Jiangsu Province in China [BK20210902]
  3. Natural Science Fund for Colleges and Universities in Jiangsu Province [21KJB180002]
  4. Innovation and Entrepreneurship Training Program for College Students in Jiangsu Province [202110313046Y, 202210313002Z]

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Glycerol is an intermediate in lipid metabolism and has been found to act as a chemical chaperone for protein conformation. This study investigates the role of glycerol in Gal-7 and demonstrates that glycerol increases the thermal stability of Gal-7 and inhibits erythrocyte agglutination. Crystal structures reveal glycerol binding sites in Gal-7, suggesting its potential as a ligand for Gal-7. Overexpression of Gal-7 also affects glycerol metabolism and triglyceride levels in cells.
Glycerol is seen in biological systems as an intermediate in lipid metabolism. In recent years, glycerol has been reported to act as a chemical chaperone to correct the conformation of proteins. Here, we investigate the role of glycerol in galectin-7 (Gal-7). The thermal shift and CD assays showed that the thermal stability of Gal-7 increased with glycerol concentration but with little secondary structure changes induced by glycerol. In addition, glycerol can inhibit Gal-7-mediated erythrocyte agglutination. We also solved the crystal structures of human Gal-7 in complex with glycerol in two different conditions. Glycerol binds at the carbohydrate-recognition binding sites of Gal-7, which indicates glycerol as a small ligand for Gal-7. Surprisingly, glycerol can bind a new pocket near the N-terminus of Gal-7, which can greatly reduce the flexibility and improve the stability of this region. Moreover, overexpression of Gal-7 decreased the intracellular triglyceride levels and increased mRNA expression of aquaporin-3 (AQP-3) when HeLa cells were incubated with glycerol. These findings indicate that Gal-7 might regulate glycerol metabolism. Overall, our results on human Gal-7 raise the perspective to systematically explore this so far unrecognized phenomenon for Gal-7 in glycerol metabolism.

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