Construction of 2DE Patterns of Plasma Proteins: Aspect of Potential Tumor Markers

The use of tumor markers aids in the early detection of cancer recurrence and prognosis. There is a hope that they might also be useful in screening tests for the early detection of cancer. Here, the question of finding ideal tumor markers, which should be sensitive, specific, and reliable, is an acute issue. Human plasma is one of the most popular samples as it is commonly collected in the clinic and provides noninvasive, rapid analysis for any type of disease including cancer. Many efforts have been applied in searching for ideal tumor markers, digging very deep into plasma proteomes. The situation in this area can be improved in two ways-by attempting to find an ideal single tumor marker or by generating panels of different markers. In both cases, proteomics certainly plays a major role. There is a line of evidence that the most abundant, so-called classical plasma proteins, may be used to generate a tumor biomarker profile. To be comprehensive these profiles should have information not only about protein levels but also proteoform distribution for each protein. Initially, the profile of these proteins in norm should be generated. In our work, we collected bibliographic information about the connection of cancers with levels of classical plasma proteins. Additionally, we presented the proteoform profiles (2DE patterns) of these proteins in norm generated by two-dimensional electrophoresis with mass spectrometry and immunodetection. As a next step, similar profiles representing protein perturbations in plasma produced in the case of different cancers will be generated. Additionally, based on this information, different test systems can be developed.

Automated summary

The use of tumor markers is important for early detection of cancer recurrence and prognosis. Finding ideal tumor markers that are sensitive, specific, and reliable is a crucial issue. Human plasma is a commonly used sample in clinics and provides noninvasive, rapid analysis for various diseases including cancer. Proteomics plays a major role in the search for ideal tumor markers. Classic plasma proteins, which are abundant, can be used to generate tumor biomarker profiles. These profiles should include information about protein levels and proteoform distribution. Future research involves generating profiles of these proteins in different cancers and developing test systems based on this information.