MicroRNA-584-5p Restrains the Viability of Lung Cancer Cells through Targeting DPY30 Directly

Dysregulation of microRNAs (miRNAs) in lung cancer participates in lung cancer progression. This study was focused on investigating regulatory mechanism of miR-584-5p in lung cancer cells. Differential miR-584-5p and DPY30 expressions in samples of lung carcinoma were evaluated using ENCORI and GEPIA (http://gepia.cancer-pku.cn/). Using RT-qPCR, miR-584-5p was detected to be downregulated in lung cancer cells. MiR-584-5p overexpression inhibited H460 cell viability and suppressing Ki67 and PCNA protein expressions. Moreover, DPY30 was found to be targeted by miR-584-5p using luciferase reporter test, which was upregulated in lung cancer cells. Furthermore, overexpressed DPY30 restrained effects of miR-584-5p mimics, causing accelerated H460 cell viabilities and upregulated Ki67 and PCNA protein expressions. In H460 cells, miR-594-5p suppressed cell viability and inhibited protein expressions of Ki67 and PCNA through binding DPY30.

Automated summary

This study revealed that miR-584-5p is downregulated and DPY30 is upregulated in lung cancer cells. Overexpression of miR-584-5p inhibits cell viability and protein expressions of Ki67 and PCNA. DPY30 is targeted by miR-584-5p and overexpressed DPY30 attenuates the effects of miR-584-5p.