期刊
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
卷 220, 期 -, 页码 1197-1211出版社
ELSEVIER
DOI: 10.1016/j.ijbiomac.2022.08.128
关键词
beta-Glucooligosaccharide; HPAEC-PAD-MS/MS; Dectin-1
资金
- Natural Science Foundation of Shanghai, China [20ZR1418700]
- Earmarked Fund for China Agriculture Research System, China [CARS-20]
- Lead- ing Talents Fund in Minhang District of Shanghai, China [201844]
- SAAS Program for Excellent Research Team [G2022003]
This study investigated the microwave-assisted degradation of beta-(1 -> 3,1 -> 6)-D-glucan from Ganoderma lucidum and its immunoregulatory activities. The degradation process yielded a high rate of degradation and abundant beta-oligosaccharide products with different degrees of polymerization. The immunoregulation activity of these beta-glucooligosaccharides was correlated with their degrees of polymerization and the ratios of beta-(1 -> 3) to beta-(1 -> 6)-linked glucose units.
Microwave-assisted degradation of beta-(1 -> 3,1 -> 6)-D-glucan from Ganoderma lucidum and correlated immunoregulatory activities were investigated in this study. The optimal temperature and degradation time for microwave hydrothermal hydrolysis were 140 degrees C and 40 min, respectively. Under these conditions, a high yield of degradation rate (98.4 %) and abundant beta-oligosaccharide products (GLOS) with different degrees of polymerization (DP 2-24) were obtained. Four fractions including F1 (DP 2-8), F2 (DP 6-19), F3 (DP 8-24) and F4 (high DPs) with different average ratios of beta-(1 -> 3) to beta-(1 -> 6)-linked glucose units were isolated from GLOS. The structures of oligosaccharides with DP (2-6) in Fl were identified as linear beta-(1 -> 3)-linked glucooligosaccharides without or with beta-(1 -> 6)-linked glucose residues based on MS/MS analysis. The immunoregulation activity of beta-glucooligosaccharides was correlated with their DPs and the average ratios of beta-(1 -> 3) to beta-(1 -> 6)- linked glucose units. F4 fraction with high DPs and ratio of 3.29:1 exhibited higher immunoenhancing activity on inducing NF-kappa B activation through binding to dectin-1. Surface plasmon resonance (SPR) analysis indicated that beta-glucooligosaccharides could bind to Dectin-1 directly and the binding affinity increased with the increase of DPs and the ratios of beta-(1 -> 3)-linked glucose.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据