期刊
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
卷 221, 期 -, 页码 558-572出版社
ELSEVIER
DOI: 10.1016/j.ijbiomac.2022.09.041
关键词
Ficolin; Portunus trituberculatus; Pattern recognition receptor; Innate immunity; Hemolymph clotting
资金
- National Key R & D Program of China [2018YFD0901304]
- National Natural Science Foundation of China [41776159]
- China Agriculture Research System - 48 [CARS -48]
In this study, a ficolin gene Ptficolin was characterized from the swimming crab Portunus trituberculatus. Ptficolin showed higher expression in stomach and hepatopancreas, and could recognize and eliminate invading pathogens, playing an important role in hemolymph coagulation in crabs.
Ficolins, belonging to the fibrinogen-related protein superfamily, are important pattern recognition receptors in innate immunity. Here, a ficolin gene Ptficolin was characterized from the swimming crab Portunus trituberculatus. The completed cDNA sequence of Ptficolin encoded a signal peptide, a coiled-coil region and a fibrinogen -like domain but without the typical collagen region of vertebrate ficolins. Ptficolin showed higher expression in stomach and hepatopancreas, and presented a time-dependent response after pathogen challenge and injury stimulation. The recombinant Ptficolin (rPtficolin) could bind to various PAMPs and microorganisms, and agglutinate microorganisms and rabbit erythrocytes in a Ca2+-dependent manner, with strong binding ability to N-acetyl sugars. Meanwhile, rPtficolin promoted the hemocyte phagocytosis and clearance activity of Vibrio, while Ptficolin knockdown impaired the bacterial phagocytosis and clearance ability, suggesting the opsonin activity of Ptficolin. Knockdown of Ptficolin could downregulate the transcription of most complement-like genes and AMPs, but enhance the expression of most proPO system-related genes and key genes of Toll, IMD and JNK pathways. Moreover, knockdown of Ptficolin led to the increased hemolymph clotting time and the decreased expression of clotting-related genes. Our results indicate that Ptficolin could recognize and eliminate invading pathogens, and might be a prominent component in hemolymph coagulation of crab.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据