Novel-miR-310 mediated response mechanism to Cry1Ac protoxin in Plutella xylostella (L.)

The diamondback moth (DBM), Plutella xylostella (L.), has evolved resistance to multiple insecticides including Bacillus thuringiensis (Bt). ATP-binding cassette (ABC) transporters are a class of transmembrane protein families, involved in multiple physiological processes and pesticide resistances in insects. However, the role and regula-tory mechanism of ABC transporter in mediating the response to Bt Cry1Ac toxin remain unclear. Here, we characterized a MAPK signaling pathway-enriched ABCG subfamily gene PxABCG20 from DBM, and found it was differentially expressed in the Cry1Ac-resistant and Cry1Ac-susceptible strains. RNAi knockdown of PxABCG20 increased the tolerance of DBM to Cry1Ac protoxin. To explore the regulatory mechanism of PxABCG20 expression, we predicted the potential miRNAs targeting PxABCG20 using two target prediction algorithms. Luciferase reporter assay confirmed that novel-miR-310 was able to down-regulate PxABCG20 expression in HEK293T cells. Furthermore, injection of novel-miR-310 agomir markedly inhibited PxABCG20 expression, resulting in increased tolerance to Cry1Ac protoxin in susceptible strain, while injection of novel-miR-310 antagomir markedly induced the expression of PxABCG20, leading to decreased tolerance to Cry1Ac protoxin. Our work provides theoretical basis for exploring novel targets for the DBM response to Cry1Ac toxin and ex-pands the understanding of miRNA role in mediating the susceptibility of insect pest to Cry1Ac toxin.

Automated summary

This study identified the ABC transporter gene PxABCG20 from diamondback moth, showing differential expression in Cry1Ac-resistant and susceptible strains, and found that RNAi knockdown of PxABCG20 increased tolerance to Cry1Ac protoxin. Furthermore, miRNA novel-miR-310 was discovered to regulate PxABCG20 expression, affecting the susceptibility of insects to Cry1Ac toxin.