4.5 Review

Advances in scaffolds used for pulp-dentine complex tissue engineering: A narrative review

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Clinical cell-based versus cell-free regenerative endodontics: clarification of concept and term

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Summary: There is confusion over the concept and term of clinical regenerative endodontics, with commonly used terms being revitalization and revascularization. The aim of regenerative endodontics is to regenerate the pulp-dentine complex, with current protocols mostly falling under the category of cell-free regenerative endodontic therapy. Repair derived from stem cells has been observed following regenerative endodontic treatments, although ideal regeneration through autologous stem cell transplantation (CB-RERT) is still at the clinical trial stage.

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Summary: Fibronectin-loaded nanofiber scaffolds were developed and evaluated for their bioactive potential on human apical papilla cells. Both random (NR) and aligned (NA) scaffolds showed good cytocompatibility, with NR and NA based on 10% PCL promoting the highest cell proliferation, adhesion, and spreading. Increasing the concentration of fibronectin led to enhanced cell migration, viability, proliferation, adhesion, collagen synthesis, and gene expression. Additionally, NA tubular scaffolds loaded with 10 µg/ml fibronectin exhibited the highest chemotactic potential on human apical papilla cells.

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Hypoxia-mimicking cobalt-doped multi-walled carbon nanotube nanocomposites enhance the angiogenic capacity of stem cells from apical papilla

Junqing Liu et al.

Summary: The study successfully prepared a novel hypoxia-mimicking nanocomposite MWCNTs/Co using the metal-organic framework method, with well-designed connecting structure. Results showed that the synthesized nanocomposites could significantly upregulate the expression of key angiogenesis proteins in stem cells, indicating promising angiogenic potential for dental tissue engineering.

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Summary: The study demonstrates the potential of acellular self-assembling peptide hydrogels in promoting tissue revascularization and soft tissue regeneration after endodontic treatment. The hydrogels show promise in guiding the development of neovasculature and tissue deposition, providing a scaffold for soft tissue regeneration in vivo.

ACTA BIOMATERIALIA (2021)

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hDPSC-laden GelMA microspheres fabricated using electrostatic microdroplet method for endodontic regeneration

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Summary: The use of GelMA microspheres as cell delivery vehicles in endodontic regeneration shows great potential due to their excellent bioactivity and biodegradability. Encapsulated dental pulp stem cells in the microspheres demonstrate functions such as adhesion, proliferation, and extracellular matrix protein secretion, leading to the generation of more vascularized pulp-like tissues in vivo.

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Summary: Current clinical evidence suggests that in regenerative endodontics, the use of PRP or PRF does not provide a significant advantage over induced blood clots. The true nature of neoformed tissues and their response to insult or injury remains poorly characterized and unexplored.

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Kuan-Liang Chen et al.

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Tiejun Qu et al.

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Hongwei Lv et al.

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