Fatty acid binding protein is required for chitin biosynthesis in the wing of Drosophila melanogaster

Chitin, the major structural polysaccharide in arthropods such as insects and mites, is a linear polymer of Nacetylglucosamine units. The growth and development of insects are intimately coupled with chitin biosynthesis. The membrane-bound beta-glycosyltransferase chitin synthase is known to catalyze the key polymerization step of N-acetylglucosamine. However, the additional proteins that might assist chitin synthase during chitin biosynthesis are not well understood. Recently, fatty acid binding protein (Fabp) has been suggested as a candidate that interacts with the chitin synthase Krotzkopf verkehrt (Kkv) in Drosophila melanogaster. Here, using split-ubiquitin membrane yeast two-hybrid and pull-down assays, we have demonstrated that the Fabp-B splice variant physically interacts with Kkv in vitro. The global knockdown of Fabp in D. melanogaster using RNA interference (RNAi) induced lethality at the larval stage. Moreover, in tissue-specific RNAi experiments, silenced Fabp expression in the epidermis and tracheal system caused a lethal larval phenotype. Fabp knockdown in the wings resulted in an abnormal wing development and uneven cuticular surface. In addition to reducing the chitin content in the first longitudinal vein of wings, Fabp silencing also caused the loss of procuticle laminate structures. This study revealed that Fabp plays an important role in chitin synthesis and contributes to a comprehensive understanding of the complex insect chitin biosynthesis.

Automated summary

Chitin is a major polysaccharide found in arthropods such as insects and mites, and its biosynthesis is not fully understood. This study reveals that fatty acid binding protein (Fabp) interacts with chitin synthase Kkv and plays an important role in chitin synthesis. Fabp knockdown in fruit flies resulted in developmental abnormalities and wing defects, indicating its significance in insect chitin biosynthesis.